Lmajor_gp63_10_0460_P460G_D463N_S465A
(Plasmid #171645)

• Purpose: Expression of L. major glcyoprotein-63 (P460G_D463N_S465A) with substrate binding site mutations from chromosome 10 in mammalian cells
• Depositing Lab: Dennis Ko
• Publication: Antonia et al PLoS Negl Trop Dis. 2021 Oct 28;15(10):e0009224. doi: 10.1371/journal.pntd.0009224. eCollection 2021 Oct.

Backbone

• Vector backbone: pcDNA3.1/Hygro(+)
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: LmjF.10.0460 P460G D463N S465A
• Species: L. major
• Mutation: P460G; D463N; S465A
• Tag / Fusion Protein:
  • Myc-His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The sequence encoding the endogenous secretion signal peptide was removed and replaced with a Kozak consensus, start codon, and secrecon signal peptide sequence.