|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17179||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Gene/Insert namenacre promoter driving nacre cDNA
SpeciesD. rerio (zebrafish)
MutationAll three Tcf/Lef sites are mutated, but 5 LEF sites are added at a new position
Entrez Genemitfa (a.k.a. nacre, z3A.1)
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Note for Use:
Description: The NacMS>Nac construct (with mutations in endogenous.
LEF sites) was engineered to have 5 LEF sites added at a new position to.
"rescue" beta-catenin responsiveness of the promoter.
Ref: R.I. Dorsky et al., 2000, Genes & Development, 14:158-162.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:M29 NacMS+LEF>Nac was a gift from Randall Moon (Addgene plasmid # 17179 ; http://n2t.net/addgene:17179 ; RRID:Addgene_17179)
Map uploaded by the depositor.