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pETDuet S2
(Plasmid #171921)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 171921 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pETDuet-1
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5267
  • Total vector size (bp) 6108
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    RNA-based (p)ppGpp sensor
  • Species
    Synthetic
  • Insert Size (bp)
    136
  • Promoter T7

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site SgrAI (not destroyed)
  • 3′ cloning site SacII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    NirFP
  • Alt name
    eqFP670
  • Species
    Synthetic
  • Insert Size (bp)
    705
  • Promoter T7

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The pETDuet vector with eqFP670 cloned was received from Samie R. Jaffrey lab at Weill Medical College, Cornell University.
  • Article Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene QC identified a C66Y change in the lacI coding sequence that may impact the leakiness of expression of the (p)ppGpp sensor and NirFP insert.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETDuet S2 was a gift from Mingxu You (Addgene plasmid # 171921 ; http://n2t.net/addgene:171921 ; RRID:Addgene_171921)
  • For your References section:

    Live-Cell Imaging of Guanosine Tetra- and Pentaphosphate (p)ppGpp with RNA-based Fluorescent Sensors*. Sun Z, Wu R, Zhao B, Zeinert R, Chien P, You M. Angew Chem Int Ed Engl. 2021 Nov 2;60(45):24070-24074. doi: 10.1002/anie.202111170. Epub 2021 Oct 5. 10.1002/anie.202111170 PubMed 34487413