Skip to main content
Addgene
Open Menu

pPIC9K_A2AR_316_V229C
(Plasmid #171940)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171940 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart

Backbone

  • Vector backbone
    pPIC9K
  • Vector type
    Yeast Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Adenosine A2A receptor
  • Species
    H. sapiens (human)
  • Mutation
    truncated to aa 1-316, V229C
  • Entrez Gene
    ADORA2A (a.k.a. A2aR, ADORA2, RDC8)
  • Promoter AOX1
  • Tags / Fusion Proteins
    • alpha-factor secretion signal, FLAG (N terminal on insert)
    • 10xHis (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPIC9K_A2AR_316_V229C was a gift from Scott Prosser & Libin Ye (Addgene plasmid # 171940 ; http://n2t.net/addgene:171940 ; RRID:Addgene_171940)

  • For your References section:

    Activation of the A2A adenosine G-protein-coupled receptor by conformational selection. Ye L, Van Eps N, Zimmer M, Ernst OP, Prosser RS. Nature. 2016 May 12;533(7602):265-8. doi: 10.1038/nature17668. Epub 2016 May 4. 10.1038/nature17668 PubMed 27144352
Related items: