pcDNA3.1-TUBG2
(Plasmid #171966)

• Purpose: Expression in cell lines of the TUBG2 gene
• Depositing Lab: Maria Alvarado-Kristensson
• Publication: Corvaisier et al Commun Biol. 2021 Jun 22;4(1):767. doi: 10.1038/s42003-021-02280-1.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: thermofisher
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 6784
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TUBG2
• Species: H. sapiens (human)
• Insert Size (bp): 1356
• Mutation: Removal of the internal TUBG2-EcoRI site by changing the nucleotide from a to g at position 117
• GenBank ID: NM_016437.3

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRV (not destroyed)
• 5′ sequencing primer: TAA TAC GAC TCA CTA TAG GG
• 3′ sequencing primer: TAGAAGGCACAGTCGAGG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: mCherry tagged TUBG2/pReceiver-M56 was purchased from GeneCopeia, Rockville, USA.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The internal EcoRI was removed from the GeneCopeia mCherry tagged TUBG2/pReceiver-M56 plasmid and TUBG2 was sub-cloned into pcDNA3.1.