pBMN-FKBP DD(L106P)-UbK0G75C-sfGFP
(Plasmid #172001)

• Purpose: Expression for LIBRON constructs
• Depositing Lab: Thomas Wandless
• Publication: Miyamae et al Cell Chem Biol. 2020 Dec 17;27(12):1573-1581.e3. doi: 10.1016/j.chembiol.2020.09.004. Epub 2020 Oct 1.

Backbone

• Vector backbone: pBMN
• Total vector size (bp): 7547
• Vector type: Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Growth instructions: Culture at 30C to prevent recombination of LTR and lost insert. Takes >20 hours to get good culture at 30C.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: FKBP DD (L106P)-UbK0G75C
• Species: H. sapiens (human)
• Insert Size (bp): 567
• Mutation: change all lysin residues of ubiquitin to Arg, G75 was substituted to Cys
• Tag / Fusion Protein:
  • sfGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: NsiI (unknown if destroyed)
• 5′ sequencing primer: pBMN 5'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry