M70 pGL3 Sp5l P1203
(Plasmid #17208)

• Depositing Lab: Randall Moon
• Publication: Other Plasmids (unpublished)

Backbone

• Vector backbone: pGL3-Basic
• Backbone manufacturer: promega
• Backbone size w/o insert (bp): 4818
• Vector type: Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Sp5l promoter
• Species: D. rerio (zebrafish)
• Insert Size (bp): 1250
• Entrez Gene: sp5l (a.k.a. Spr2, YF-335, cb577, fc23b01, s, sb:cb57, sb:cb577, wu:fc23b01)
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: LucNRev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Description: a fragment of putative zebrafish Sp5l promoter - 1203bp upstream of the 1st Exon (as defined by the 27i2 clone found in the microarray screen) - plus 51bp of 5'UTR from Exon1 cloned upstream of firefly luciferase in the pGL3 Basic promoter. For testing whether this fragment is Wnt responsive. Amplified from KWT genomic DNA using oligos 328 & 329, cloned XhoI / HindIII in pGL3-Basic (from Ajamete). Sequenced using 328 & 329 (ergo junctions not sequenced); 2 SNPs to Ensembl sequence.