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M70 pGL3 Sp5l P1203
(Plasmid #17208)


Item Catalog # Description Quantity Price (USD)
Plasmid 17208 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4818
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    Sp5l promoter
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
  • Entrez Gene
    sp5l (a.k.a. Spr2, YF-335, cb577, fc23b01, s, sb:cb57, sb:cb577, wu:fc23b01)
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer LucNRev
  • (Common Sequencing Primers)

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Description: a fragment of putative zebrafish Sp5l promoter - 1203bp upstream of the 1st Exon (as defined by the 27i2 clone found in the microarray screen) - plus 51bp of 5'UTR from Exon1 cloned upstream of firefly luciferase in the pGL3 Basic promoter. For testing whether this fragment is Wnt responsive. Amplified from KWT genomic DNA using oligos 328 & 329, cloned XhoI / HindIII in pGL3-Basic (from Ajamete). Sequenced using 328 & 329 (ergo junctions not sequenced); 2 SNPs to Ensembl sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M70 pGL3 Sp5l P1203 was a gift from Randall Moon (Addgene plasmid # 17208 ; ; RRID:Addgene_17208)