CPL2g/pDONR221
(Plasmid #172093)

• Purpose: Genomic fragment containing the upstream region and coding region of CER11/CPL2 cloned into pDONR221
• Depositing Lab: George Haughn
• Publication: Shi et al Plant Physiol. 2019 Nov;181(3):901-915. doi: 10.1104/pp.19.00722. Epub 2019 Sep 4.

Backbone

• Vector backbone: pDONR221
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4761
• Total vector size (bp): 11531
• Vector type: Gateway Entry vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CPL2
• Alt name: CER11
• Species: A. thaliana (mustard weed)
• Entrez Gene: CPL2 (a.k.a. AT5G01270, ATCPL2, F7J8.250, F7J8_250, carboxyl-terminal domain (ctd) phosphatase-like 2)
• Promoter: CPL2

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13For
• 3′ sequencing primer: M13Rev

Resource Information

• Supplemental Documents:
  • CPL2g fragment from pDONR221

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Note that the location of the stop codon based on TAIR gene model AT5G01270.1