6xHis-CPL2/pET28a
(Plasmid #172095)

• Purpose: cDNA of CER11/CPL2 cloned into pET28a for protein expression
• Depositing Lab: George Haughn
• Publication: Shi et al Plant Physiol. 2019 Nov;181(3):901-915. doi: 10.1104/pp.19.00722. Epub 2019 Sep 4.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: EMD Biosciences
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 7676
• Modifications to backbone: NA
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CPL2
• Alt name: CER11
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 2313
• GenBank ID: NM_120205
• Entrez Gene: CPL2 (a.k.a. AT5G01270, ATCPL2, F7J8.250, F7J8_250, carboxyl-terminal domain (ctd) phosphatase-like 2)
• Promoter: T7
• Tag / Fusion Protein:
  • 6x His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: T7 Promoter
• 3′ sequencing primer: T7 Terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA