|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17214||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 3934
Vector typeBacterial Expression
Growth in Bacteria
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Bacterial co-expression vectors to facilitate the production of substantial amounts of recombinant multiprotein complexes for biochemical, biophysical, and structural studies. pOKD4 (kanamycin-resistant) and pOKD5 (ampicillin-resistant) are derivatives of pACYC177 cloning and pET26b expression vectors. As a result, pOKD4 and pOKD5 are T7-based expression plasmids containing the p15A origin of replication. This feature permits either pOKD4 or pOKD5 to co-exist in the same bacterial cell with most Escherichia coli expression vectors including the popular pET expression vectors.
Full sequence is approximated. See article for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pOKD4 was a gift from Hao Wu (Addgene plasmid # 17214 ; http://n2t.net/addgene:17214 ; RRID:Addgene_17214)
For your References section:General co-expression vectors for the overexpression of heterodimeric protein complexes in Escherichia coli. Dzivenu OK, Park HH, Wu H. Protein Expr Purif. 2004 Nov . 38(1):1-8. 10.1016/j.pep.2004.07.016 PubMed 15477075