pBAD-MtrxRFP2
(Plasmid
#172323)
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Purposefluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 172323 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBAD/HisB
- Total vector size (bp) 5273
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namehuman thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
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Alt nameMtrxRFP2
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SpeciesSynthetic
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Insert Size (bp)1317
- Promoter pBAD
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Tag
/ Fusion Protein
- His-tag (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pBAD-Forward
- 3′ sequencing primer pBAD-Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBAD-MtrxRFP2 was a gift from Huiwang Ai (Addgene plasmid # 172323 ; http://n2t.net/addgene:172323 ; RRID:Addgene_172323) -
For your References section:
Improved Red Fluorescent Redox Indicators for Monitoring Cytosolic and Mitochondrial Thioredoxin Redox Dynamics. Pang Y, Zhang H, Ai HW. Biochemistry. 2022 Mar 1;61(5):377-384. doi: 10.1021/acs.biochem.1c00634. Epub 2022 Feb 8. 10.1021/acs.biochem.1c00634 PubMed 35133140
