pbZIP9:GFP-GUS
(Plasmid #172483)

• Purpose: GFP-GUS driven by the bZIP9 promoter
• Depositing Lab: Wolf Frommer
• Publication: Kim et al Plant Cell. 2021 May 5;33(3):511-530. doi: 10.1093/plcell/koaa060.

Backbone

• Vector backbone: pBGWFS7,0
• Backbone manufacturer: 12454
• Backbone size w/o insert (bp): 10703
• Total vector size (bp): 12454
• Vector type: Plant Expression
• Selectable markers: Basta

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: bZIP9 promoter
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 1595
• Entrez Gene: BZIP9 (a.k.a. AT5G24800, ARABIDOPSIS THALIANA BASIC LEUCINE ZIPPER 9, ATBZIP9, BASIC LEUCINE ZIPPER O2 HOMOLOG 2, BZIP PROTEIN BZO2H2, BZO2H2, F6A4.10, F6A4_10, basic leucine zipper 9)
• Tag / Fusion Protein:
  • GFP and GUS (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: aaatgaggacgttgcattgac
• 3′ sequencing primer: CAGCTCCTCGCCCTTGCTCA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains I60F, Q279E, and V420-P426 mutations in GUS. These mutations are not known to affect plasmid function as the plasmid was functional for depositor's experiments.