MAC-N-GFP
(Plasmid
#172974)
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PurposeMAC-tagged GFP expression
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 172974 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneMAC-tag-C
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Vector typeMammalian Expression, Bacterial Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP
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Alt nameGreen fluorescent protein
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SpeciesAequorea victoria
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GenBank IDAAA27722.1
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Tag
/ Fusion Protein
- MAC-tag (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: this plasmid contains a Y238F mutation in GFP. This mutation does not affect the plasmid's function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MAC-N-GFP was a gift from Markku Varjosalo (Addgene plasmid # 172974 ; http://n2t.net/addgene:172974 ; RRID:Addgene_172974) -
For your References section:
Combined proximity labeling and affinity purification-mass spectrometry workflow for mapping and visualizing protein interaction networks. Liu X, Salokas K, Weldatsadik RG, Gawriyski L, Varjosalo M. Nat Protoc. 2020 Aug 10. pii: 10.1038/s41596-020-0365-x. doi: 10.1038/s41596-020-0365-x. 10.1038/s41596-020-0365-x PubMed 32778839
