pMito-HyPerGRt
(Plasmid
#173197)
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PurposeHyPer7 and tdTomato fusion to detect mitochondrial hydrogen peroxide
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Depositing Lab
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Depositing OrganizationUniversity of Virginia
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 173197 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 173197-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 173197-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4088
- Total vector size (bp) 7178
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHyper7-tdTomato
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Alt nameHyPerGRT
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SpeciesSynthetic
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Insert Size (bp)3089
- Promoter CMV IE94 promoter
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH I (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer tacaagctacttgttctttttgcag
- 3′ sequencing primer tggatctacgtaatacgactcactata
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 173197-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 173197-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMito-HyPerGRt was a gift from Huiwang Ai (Addgene plasmid # 173197 ; http://n2t.net/addgene:173197 ; RRID:Addgene_173197) -
For your References section:
Ratiometric Imaging of Mitochondrial Hydrogen Peroxide in Abeta42-Mediated Neurotoxicity. Li X, Zhang Y, Ai HW. ACS Sens. 2022 Feb 28. doi: 10.1021/acssensors.1c01381. 10.1021/acssensors.1c01381 PubMed 35226474
