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Purposemammalian expression of ATeam1.03-nD/nA, a FRET-based ATP indicator
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 51958 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameATeam1.03-nD/nA
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SpeciesSynthetic
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Insert Size (bp)1800
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ATeam1.03-nD/nA/pcDNA3 was a gift from Takeharu Nagai (Addgene plasmid # 51958 ; http://n2t.net/addgene:51958 ; RRID:Addgene_51958) -
For your References section:
Reversible dimerization of Aequorea victoria fluorescent proteins increases the dynamic range of FRET-based indicators. Kotera I, Iwasaki T, Imamura H, Noji H, Nagai T. ACS Chem Biol. 2010 Feb 19;5(2):215-22. doi: 10.1021/cb900263z. 10.1021/cb900263z PubMed 20047338
Map uploaded by the depositor.
