PurposePyronic, FRET sensor that allow quantitative measurement of pyruvate transport, pyruvate production and mitochondrial pyruvate consumption in intact individual cells at high temporal resolution.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51308||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5419
- Total vector size (bp) 7681
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418), Hygromycin
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)2262
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7Promoter
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Pyronic/pcDNA3.1(-) was a gift from Luis Felipe Barros (Addgene plasmid # 51308 ; http://n2t.net/addgene:51308 ; RRID:Addgene_51308)
For your References section:Imaging Mitochondrial Flux in Single Cells with a FRET Sensor for Pyruvate. San_Martin A, Ceballo S, Baeza-Lehnert F, Lerchundi R, Valdebenito R, Contreras-Baeza Y, Alegria K, Barros LF. PLoS One. 2014 Jan 21;9(1):e85780. doi: 10.1371/journal.pone.0085780. eCollection 2014 Jan 21. PubMed 24465702