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pPiggyBacATCC LacZ GCTT loxAttP
(Plasmid #173496)


Item Catalog # Description Quantity Price (USD)
Plasmid 173496 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pDONR221 P4r-P3r
  • Backbone manufacturer
  • Backbone size (bp) 2200
  • Modifications to backbone
    addition of piggyBac transposon 5' and 3' terminal repeats flanking: - one attP docking site from phage PhiC31 - a loxP cassette encompassing LacZ (for blue white selection), LacZ is flanked by two BsaI sites for Golden Gate Cloning.
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Golden Gate assemble your genes of interest using the type IIs BsaI (or Eco31I) restriction enzyme, which generates a
TAGG-5' overhang on one side, and a 5'-GCTT overhang on the other side of the vector. In our protocols these overhangs are termed: ATCC, GCTT (only considering the upper DNA strand for simplicity).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPiggyBacATCC LacZ GCTT loxAttP was a gift from Eric Marois (Addgene plasmid # 173496 ; ; RRID:Addgene_173496)
  • For your References section:

    Transgenic expression of Nix converts genetic females into males and allows automated sex sorting in Aedes albopictus. Lutrat C, Olmo RP, Baldet T, Bouyer J, Marois E. Commun Biol. 2022 Mar 7;5(1):210. doi: 10.1038/s42003-022-03165-7. 10.1038/s42003-022-03165-7 PubMed 35256751