Arp2/3_pBIG2abcd
(Plasmid #173683)

• Purpose: Insect expression of the 7-subunit Human Arp2/3 complex
• Depositing Lab: Roberto Dominguez
• Publication: Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaaz7651. doi: 10.1126/sciadv.aaz7651. Print 2020 Jun.

Backbone

• Vector backbone: pBIG2abcd
• Backbone size w/o insert (bp): 5668
• Total vector size (bp): 16996
• Vector type: Insect Expression
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Arp2
• Species: H. sapiens (human)
• Insert Size (bp): 1185
• Promoter: polyhedrin

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: Arp2_seq_for: ATGGATTCGCAGGGCCG
• 3′ sequencing primer: Arp2_seq_rev: TTATCTTACAGTAACACCGAGTTTTTCGAG

Gene/Insert 2

• Gene/Insert name: Arp3
• Species: H. sapiens (human)
• Insert Size (bp): 1254
• Promoter: polyhedrin

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: Arp3_seq_for: ATGGCGGGACGCTTGC
• 3′ sequencing primer: Arp3_seq_rev: TTAGGACATGACGCCAAACACG

Gene/Insert 3

• Gene/Insert name: ArpC2
• Species: H. sapiens (human)
• Insert Size (bp): 900
• Promoter: polyhedrin

Cloning Information for Gene/Insert 3

• Cloning method: Gibson Cloning
• 5′ sequencing primer: -
• 3′ sequencing primer: ArpC2_seq_rev: TTAGCGTGAGGAGAAAGTTTTGCC

Gene/Insert 4

• Gene/Insert name: ArpC3-FLAG
• Species: H. sapiens (human)
• Insert Size (bp): 594
• Promoter: polyhedrin

Cloning Information for Gene/Insert 4

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ArpC3_seq_for: ATGCCCGCATACCACTCTTC

Gene/Insert 5

• Gene/Insert name: ArpC4
• Species: H. sapiens (human)
• Insert Size (bp): 504
• Promoter: polyhedrin

Cloning Information for Gene/Insert 5

• Cloning method: Gibson Cloning
• 5′ sequencing primer: -
• 3′ sequencing primer: ArpC4_seq_rev: TTAAAAATTTTTCAGGAACTCTTCGGCAAC

Gene/Insert 6

• Gene/Insert name: ArpC5
• Species: H. sapiens (human)
• Insert Size (bp): 456
• Promoter: polyhedrin

Cloning Information for Gene/Insert 6

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ArpC5_seq_rev: TTATACGGTTTTTCTCGCAGTAAGAACG

Gene/Insert 7

• Gene/Insert name: ArpC1
• Species: H. sapiens (human)
• Insert Size (bp): 1116
• Promoter: polyhedrin

Cloning Information for Gene/Insert 7

• Cloning method: Gibson Cloning
• 5′ sequencing primer: -
• 3′ sequencing primer: ArpC1_seq_rev: TTATTTAATTTTCAAATCTTTGAGGGCTGATTCG

Resource Information

• Supplemental Documents:
  • Arp23_pBIG2abcd.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry