pJRK261
(Plasmid
#173754)
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PurposeCeLINC plasmid
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 173754 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepMLS257
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Backbone manufacturerAgilent Technologies
- Backbone size w/o insert (bp) 2999
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name(empty)::CRY2(olig)::VHH(GFP)::T2A::CIBN-3xFLAG::MP::unc-54 3' UTR
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SpeciesC. elegans (nematode)
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Insert Size (bp)3660
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJRK261 was a gift from Mike Boxem (Addgene plasmid # 173754 ; http://n2t.net/addgene:173754 ; RRID:Addgene_173754) -
For your References section:
CeLINC, a fluorescence-based protein-protein interaction assay in Caenorhabditis elegans. Kroll JR, Remmelzwaal S, Boxem M. Genetics. 2021 Dec 10;219(4). pii: 6380436. doi: 10.1093/genetics/iyab163. 10.1093/genetics/iyab163 PubMed 34849800
