Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17377||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepBS KS(+)
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)1300
Entrez GeneWnt5b (a.k.a. AW545702, Wnt-5b)
/ Fusion Protein
- HA (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Expression of multiple novel Wnt-1/int-1-related genes during fetal and adult mouse development.
Gavin BJ, McMahon JA, McMahon AP
In Genes Dev. 1990 Dec . 4(12B):2319-32.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBS Wnt-5bHA was a gift from Jan Kitajewski (Addgene plasmid # 17377 ; http://n2t.net/addgene:17377 ; RRID:Addgene_17377)
For your References section:Transformation by Wnt family proteins correlates with regulation of beta-catenin. Shimizu H, Julius MA, Giarre M, Zheng Z, Brown AM, Kitajewski J. Cell Growth Differ. 1997 Dec . 8(12):1349-58. PubMed 9419423