XLone-GvpB
(Plasmid #173798)

• Purpose: Mammalian acoustic reporter gene 1 cassette 1 (expressing GvpB)
• Depositing Lab: Xiaojun Lian
• Publication: Howells et al Bioeng Transl Med. 2023 Aug 2;9(2):e10584. doi: 10.1002/btm2.10584. eCollection 2024 Mar.

Backbone

• Vector backbone: XLone
• Backbone manufacturer: Xiaojun Lian
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GvpB
• Promoter: TRE3G promoter
• Tag / Fusion Protein:
  • mCherry (K187N -please see depositor comments) (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn1 (not destroyed)
• 3′ cloning site: Spe1 (not destroyed)
• 5′ sequencing primer: M13 fwd
• 3′ sequencing primer: M13 Rvs

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note there is a single mutation K187N in mCherry. Depositor confirms this does not affect plasmid function.