pUFlip-floxed2A-KalTA4-; gcry1:BFP -2
PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||173891||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerKarl J. Clark
- Backbone size w/o insert (bp) 2730
- Total vector size (bp) 7495
Vector typeSynthetic Biology
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert name2A-KalTA4; gcry1: BFP
Alt nameP2A-monomeric KalTA4 gamma crystallin 1: BFP
SpeciesD. rerio (zebrafish)
Insert Size (bp)4765
- Cloning method Gibson Cloning
- 5′ sequencing primer aaaagcaagaaagaaaactagagtgg
- 3′ sequencing primer atggctcataacaccccttg (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note PacCI sites have replaced the BfuAI sites within the initial versions of these vectors created by the Essner lab.
Please visit https://www.biorxiv.org/content/10.1101/2021.06.18.448732v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUFlip-floxed2A-KalTA4-; gcry1:BFP -2 was a gift from Jeffrey Essner (Addgene plasmid # 173891 ; http://n2t.net/addgene:173891 ; RRID:Addgene_173891)
For your References section:Cre/lox regulated conditional rescue and inactivation with zebrafish UFlip alleles generated by CRISPR-Cas9 targeted integration. Liu F, Kambakam S, Almeida MP, Ming Z, Welker JM, Wierson WA, Schultz-Rogers LE, Ekker SC, Clark KJ, Essner JJ, McGrail M. Elife. 2022 Jun 17;11. pii: 71478. doi: 10.7554/eLife.71478. 10.7554/eLife.71478 PubMed 35713402