pSNRW-Z3-Nluc
(Plasmid #174486)

• Purpose: Recombinant fusion protein gene combining IgG-Fc binding domain Z (3 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.
• Depositing Lab: Richard C. Willson
• Publication: Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 10.1016/j.ab.2022.114929.

Backbone

• Vector backbone: pET28a
• Backbone size w/o insert (bp): 5292
• Total vector size (bp): 6352
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Z3-Nluc
• Species: Staphylococcus aureus, Oplophorus gracilirostris
• Insert Size (bp): 1065
• GenBank ID: AFI79290.1 41071997
• Promoter: T7lac promoter
• Tag / Fusion Protein:
  • 6x Histidine tag (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CGCGGCAGCCATATG
• 3′ sequencing primer: GTGGTGGTGGTGGTGC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • NanoLuc Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry