PylT(UAG) PylS mCherry-P2A-eGFP(150TAG)
(Plasmid
#174526)
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PurposeDual-fluorescence reporter for quantifying PylT(UAG) decoding efficiency
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 174526 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonePB53
- Total vector size (bp) 10600
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePylS
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Alt nameMmPylRS
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SpeciesMethanosarcina mazei
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Insert Size (bp)1395
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GenBank IDNC_003901.1
- Promoter EF-1α
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer GGAGCCTACCTAGACTCAG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PylT(UAG) PylS mCherry-P2A-eGFP(150TAG) was a gift from Yu-Hsuan Tsai (Addgene plasmid # 174526 ; http://n2t.net/addgene:174526 ; RRID:Addgene_174526) -
For your References section:
Development of mammalian cell logic gates controlled by unnatural amino acids. Mills EM, Barlow VL, Jones AT, Tsai YH. Cell Rep Methods. 2021 Sep 16;1(6):100073. doi: 10.1016/j.crmeth.2021.100073. eCollection 2021 Oct 25. 10.1016/j.crmeth.2021.100073 PubMed 35474893
