2xmNeonGreen-hMeikin
(Plasmid
#174704)
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PurposeStable expression of human Meikin
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 174704 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBABE-Blast
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Vector typeMammalian Expression, Retroviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMeikin
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SpeciesH. sapiens (human)
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Tag
/ Fusion Protein
- 2xmNeonGreen (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pNM481 . Please visit https://doi.org/10.1101/2020.11.29.402537 for bioRxiv preprint
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
2xmNeonGreen-hMeikin was a gift from Iain Cheeseman (Addgene plasmid # 174704 ; http://n2t.net/addgene:174704 ; RRID:Addgene_174704) -
For your References section:
Separase cleaves the kinetochore protein Meikin at the meiosis I/II transition. Maier NK, Ma J, Lampson MA, Cheeseman IM. Dev Cell. 2021 Aug 9;56(15):2192-2206.e8. doi: 10.1016/j.devcel.2021.06.019. Epub 2021 Jul 30. 10.1016/j.devcel.2021.06.019 PubMed 34331869
