pAS_4xMmaPylT_EF1_CRFR1-A95TAG-HA
(Plasmid #174900)

• Purpose: amber suppression mediated CRFR1 A95TAG expression in mammalian cells; transient or piggy bac mediated integration
• Depositing Lab: Simon Elsaesser
• Publication: Meineke et al Front Chem. 2021 Nov 11;9:768535. doi: 10.3389/fchem.2021.768535. eCollection 2021.

Backbone

• Vector backbone: pAS
• Backbone manufacturer: Addgene #140008 based on PB510B-1 (System Bioscience)
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CRFR1
• Alt name: Crhr1
• Species: R. norvegicus (rat)
• Insert Size (bp): 1245
• Mutation: A95TAG
• Entrez Gene: Crhr1 (a.k.a. CRF-R, CRF-R-1, CRF-R1, CRF1, CRFR-1, CRFR1, CRH-R, CRH-R 1, CRH-R-1, CRH-R1)
• Promoter: EF1
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • pas_4xmmapylt_ef1_crfr1-a95tag-ha.gb
• A portion of this plasmid was derived from a plasmid made by: CRFR1 A95TAG amber suppression in Coin et al. 2013, PMID: 24290358

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry