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(Plasmid #174900)


Item Catalog # Description Quantity Price (USD)
Plasmid 174900 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Addgene #140008 based on PB510B-1 (System Bioscience)
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
  • Mutation
  • Entrez Gene
    Crhr1 (a.k.a. CRF-R, CRF-R-1, CRF-R1, CRF1, CRFR-1, CRFR1, CRH-R, CRH-R 1, CRH-R-1, CRH-R1)
  • Promoter EF1
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    CRFR1 A95TAG amber suppression in Coin et al. 2013, PMID: 24290358

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAS_4xMmaPylT_EF1_CRFR1-A95TAG-HA was a gift from Simon Elsaesser (Addgene plasmid # 174900 ; ; RRID:Addgene_174900)
  • For your References section:

    A Genetically Encoded Picolyl Azide for Improved Live Cell Copper Click Labeling. Meineke B, Heimgartner J, Craig AJ, Landreh M, Moodie LWK, Elsasser SJ. Front Chem. 2021 Nov 11;9:768535. doi: 10.3389/fchem.2021.768535. eCollection 2021. 10.3389/fchem.2021.768535 PubMed 34858945