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Addgene

pET30(a)-AtNUDX23
(Plasmid #175021)

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Item Catalog # Description Quantity Price (USD)
Plasmid 175021 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET30a(+)
  • Backbone manufacturer
    GenScript
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AtNUDX23
  • Alt name
    Arabidopsis FAD pyrophosphohydrolase 23
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    648
  • Entrez Gene
    NUDX23 (a.k.a. AT2G42070, ARABIDOPSIS THALIANA NUDIX HYDROLASE HOMOLOG 23, ATNUDT23, ATNUDX23, T6D20.4, T6D20_4, nudix hydrolase homolog 23)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET30(a)-AtNUDX23 was a gift from Jeppe Vinther (Addgene plasmid # 175021 ; http://n2t.net/addgene:175021 ; RRID:Addgene_175021)

  • For your References section:

    Hepatitis C virus RNA is 5'-capped with flavin adenine dinucleotide. Sherwood AV, Rivera-Rangel LR, Ryberg LA, Larsen HS, Anker KM, Costa R, Vagbo CB, Jakljevic E, Pham LV, Fernandez-Antunez C, Indrisiunaite G, Podolska-Charlery A, Grothen JER, Langvad NW, Fossat N, Offersgaard A, Al-Chaer A, Nielsen L, Kusnierczyk A, Solund C, Weis N, Gottwein JM, Holmbeck K, Bottaro S, Ramirez S, Bukh J, Scheel TKH, Vinther J. Nature. 2023 Jul;619(7971):811-818. doi: 10.1038/s41586-023-06301-3. Epub 2023 Jul 5. 10.1038/s41586-023-06301-3 PubMed 37407817
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