|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17530||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI, EcoRI, MluI, KpnI (not destroyed)
- 3′ cloning site NotI, XbaI, SalI, SmaI, NotI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
The lox-targeting plasmid, plox, was generated by subcloning the Sal I (blunted)-Hind III fragment of pPGK-loxP-Xist (Wutz et al., 2002) into Bgl II (blunted)-Hind III cut pNeoEGFP (Clontech). plox has a stuffer fragment (the EGFP gene) derived from pNeoEGFP, bounded by multiple cloning sites upstream and downstream.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pPGK-loxP-neoEGFP (plox) was a gift from George Daley (Addgene plasmid # 17530)
For your References section:HoxB4 confers definitive lymphoid-myeloid engraftment potential on embryonic stem cell and yolk sac hematopoietic progenitors. Kyba M, Perlingeiro RC, Daley GQ. Cell. 2002 Apr 5. 109(1):29-37. 10.1016/S0092-8674(02)00680-3 PubMed 11955444
Map uploaded by the depositor.