pDN526
(Plasmid
#175372)
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Purpose(Empty Backbone) Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, URA3-selected.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 175372 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDN526
- Backbone size (bp) 5742
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid was generated by modification of plasmid pRS406 (Sikorski & Hieter, 1989, Genetics 122:19-27) by insertion of 2 micron high copy S. cerevisiae replication and partitioning locus with flanking restriction enzyme cut sites (AatII, AvrII, and SphI).
bioRxiv pre-print 367219: Rapid conversion of replicating and integrating Saccharomyces cerevisiae plasmid vectors via Cre recombinase
https://doi.org/10.1101/2020.11.03.367219
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDN526 was a gift from Daniel Nickerson (Addgene plasmid # 175372 ; http://n2t.net/addgene:175372 ; RRID:Addgene_175372) -
For your References section:
Rapid conversion of replicating and integrating Saccharomyces cerevisiae plasmid vectors via Cre recombinase. Nickerson DP, Quinn MA, Milnes JM. G3 (Bethesda). 2021 Dec 8;11(12). pii: 6380430. doi: 10.1093/g3journal/jkab336. 10.1093/g3journal/jkab336 PubMed 34599813
Map uploaded by the depositor.
