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(Plasmid #175523)


Item Catalog # Description Quantity Price (USD)
Plasmid 175523 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    LAMP1-HaloTag11 and Tomm20-HaloTag9
  • Alt name
  • Alt name
  • Species
    H. sapiens (human)
  • Mutation
    HaloTag11 = HaloTag7-M175W and HaloTag9 = HaloTag7-Q165H-P174R

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5/FRT/TO_LAMP1-HaloTag11-T2A-Tomn20-HaloTag9 was a gift from Kai Johnsson (Addgene plasmid # 175523 ; ; RRID:Addgene_175523)
  • For your References section:

    Engineered HaloTag variants for fluorescence lifetime multiplexing. Frei MS, Tarnawski M, Roberti MJ, Koch B, Hiblot J, Johnsson K. Nat Methods. 2021 Dec 16. pii: 10.1038/s41592-021-01341-x. doi: 10.1038/s41592-021-01341-x. 10.1038/s41592-021-01341-x PubMed 34916672