pSP72-RBM8A-3'UTR AluY-Sz WT 2598-4066
(Plasmid
#175587)
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Purposein vitro transcription of wt RBM8A 3'UTR
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 175587 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepALTER
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 2462
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Vector typein vitro transcription
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRBM8A-3'UTR AluY-Sz WT
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Insert Size (bp)1467
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GenBank ID9939
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Acc1 (not destroyed)
- 3′ cloning site SacI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSP72-RBM8A-3'UTR AluY-Sz WT 2598-4066 was a gift from Emanuela Felley-Bosco (Addgene plasmid # 175587 ; http://n2t.net/addgene:175587 ; RRID:Addgene_175587) -
For your References section:
Double-Stranded RNA Structural Elements Holding the Key to Translational Regulation in Cancer: The Case of Editing in RNA-Binding Motif Protein 8A. Abukar A, Wipplinger M, Hariharan A, Sun S, Ronner M, Sculco M, Okonska A, Kresoja-Rakic J, Rehrauer H, Qi W, Beusechem VWV, Felley-Bosco E. Cells. 2021 Dec 15;10(12). pii: cells10123543. doi: 10.3390/cells10123543. 10.3390/cells10123543 PubMed 34944051
