pLX304-DCK*-GW-IRES-GFP
(Plasmid #176289)

• Purpose: Gateway vector for use in generating DCK*-POI fusion reporter
• Depositing Lab: William Kaelin
• Publication: Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6263. doi: 10.1126/sciadv.abd6263. Print 2021 Feb.

Backbone

• Vector backbone: pLX304
• Backbone manufacturer: None
• Backbone size w/o insert (bp): 11000
• Total vector size (bp): 12000
• Modifications to backbone: an IRES-GFP cassette was sub cloned into the NheI-AgeI site. To maintain frame between the DCK* suicide gene, and the gene of interest cloned into the Gateway cassette, please make sure to be in-frame with the TACAAAAAA in the attR1 site, and the TACAAAAAA in the attR2 site.
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): ccdB Survival
• Growth instructions: Longer growth may be needed
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A)
• Species: H. sapiens (human)
• Insert Size (bp): 778
• Mutation: S74E R104M D133A
• GenBank ID: NM_000788.3
• Entrez Gene: DCK
• Tag / Fusion Protein:
  • V5 (C terminal on backbone)

Resource Information

• Supplemental Documents:
  • MAPpLX304-dCK-linker-gate-V5.gb
• A portion of this plasmid was derived from a plasmid made by: Mutant deoxycytidine kinase was the kind gift of the Medin Lab

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry