PurposeCRISPR donor plasmid to create GFP fusion proteins
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||176367||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GenePOU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)
/ Fusion Protein
- GFP (C terminal on insert)
- Cloning method Gibson Cloning
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Cloning source: WTC11 cells. Our lab normally uses Top10 cells for transformation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pPOU5F1-donor was a gift from Barbara Stranger & Kevin White (Addgene plasmid # 176367 ; http://n2t.net/addgene:176367 ; RRID:Addgene_176367)