Yiplac211 proGAL1-FRB-RGG-GFP-RGG-RGG
(Plasmid
#176400)
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PurposeExpresses GFP tagged triple RGG scaffold with N-terminal FRB. Expression induced in media containing galactose.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 176400 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneYiplac211
- Total vector size (bp) 6955
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Modifications to backboneAddition of GAL1 promoter.
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFRB-RGG-GFP-RGG-RGG
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)2547
- Promoter GAL1
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Tag
/ Fusion Protein
- FRB and GFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site AgeI (not destroyed)
- 5′ sequencing primer M13R
- 3′ sequencing primer M13F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Yiplac211 proGAL1-FRB-RGG-GFP-RGG-RGG was a gift from Matthew Good (Addgene plasmid # 176400 ; http://n2t.net/addgene:176400 ; RRID:Addgene_176400) -
For your References section:
Designer membraneless organelles sequester native factors for control of cell behavior. Garabedian MV, Wang W, Dabdoub JB, Tong M, Caldwell RM, Benman W, Schuster BS, Deiters A, Good MC. Nat Chem Biol. 2021 Sep;17(9):998-1007. doi: 10.1038/s41589-021-00840-4. Epub 2021 Aug 2. 10.1038/s41589-021-00840-4 PubMed 34341589
