pCRISPR01-FnCpf1
(Plasmid #176529)

• Purpose: Nuclease Cpf1 adapted for Aspergillus species
• Depositing Lab: Kusol Pootanakit
• Publication: Abdulrachman et al BMC Biotechnol. 2021 Feb 11;21(1):15. doi: 10.1186/s12896-021-00669-8.

Backbone

• Vector backbone: pFC333
• Backbone manufacturer: Uffe Mortensen (Addgene plasmid # 87844)
• Total vector size (bp): 12879
• Vector type: CRISPR ; Fungal Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FnCpf1
• Species: Synthetic; Francisella tularensis subsp. novicida U112
• Insert Size (bp): 3900
• Promoter: TEF1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (unknown if destroyed)
• 3′ cloning site: PmeI (unknown if destroyed)
• 5′ sequencing primer: Seq TEF F: 5'-GCCCAAGGTGGGAGTCTAGGAG
• 3′ sequencing primer: seq TEF R: 5'-CGCCTGGACGACTAAACCAAAATAGG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: synthetic

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry