pCRISPR01-FnCpf1
(Plasmid
#176529)
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PurposeNuclease Cpf1 adapted for Aspergillus species
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Depositing Lab
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Depositing OrganizationMahidol University
Located in Thailand -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 176529 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 176529-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 176529-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepFC333
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Backbone manufacturerUffe Mortensen (Addgene plasmid # 87844)
- Total vector size (bp) 12879
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Vector typeCRISPR ; Fungal Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFnCpf1
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SpeciesSynthetic; Francisella tularensis subsp. novicida U112
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Insert Size (bp)3900
- Promoter TEF1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (unknown if destroyed)
- 3′ cloning site PmeI (unknown if destroyed)
- 5′ sequencing primer Seq TEF F: 5'-GCCCAAGGTGGGAGTCTAGGAG
- 3′ sequencing primer seq TEF R: 5'-CGCCTGGACGACTAAACCAAAATAGG
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bysynthetic
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 176529-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 176529-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCRISPR01-FnCpf1 was a gift from Kusol Pootanakit (Addgene plasmid # 176529 ; http://n2t.net/addgene:176529 ; RRID:Addgene_176529) -
For your References section:
Development of a CRISPR/Cpf1 system for targeted gene disruption in Aspergillus aculeatus TBRC 277. Abdulrachman D, Eurwilaichitr L, Champreda V, Chantasingh D, Pootanakit K. BMC Biotechnol. 2021 Feb 11;21(1):15. doi: 10.1186/s12896-021-00669-8. 10.1186/s12896-021-00669-8 PubMed 33573639
