pHS0578 CRISPR associated IscB non-coding locus in pColA-Duet
(Plasmid
#176535)
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PurposeNon-protein coding locus components from CRISPR-associated IscB
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 176535 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCOLADuet1
- Backbone size w/o insert (bp) 3719
- Total vector size (bp) 6221
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namenon-coding locus components from Delaware Bay metagenome CRISPR-associated IscB
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SpeciesDelaware Bay metagenome
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Insert Size (bp)2503
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHS0578 CRISPR associated IscB non-coding locus in pColA-Duet was a gift from Feng Zhang (Addgene plasmid # 176535 ; http://n2t.net/addgene:176535 ; RRID:Addgene_176535) -
For your References section:
The widespread IS200/IS605 transposon family encodes diverse programmable RNA-guided endonucleases. Altae-Tran H, Kannan S, Demircioglu FE, Oshiro R, Nety SP, McKay LJ, Dlakic M, Inskeep WP, Makarova KS, Macrae RK, Koonin EV, Zhang F. Science. 2021 Oct;374(6563):57-65. doi: 10.1126/science.abj6856. Epub 2021 Sep 9. 10.1126/science.abj6856 PubMed 34591643
