pHS0806 His14-bdSUMO-AwaIscB
(Plasmid #176539)

• Purpose: Bacterial expression of His14-bdSUMO-AwaIscB
• Depositing Lab: Feng Zhang
• Publication: Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi: 10.1126/science.abj6856. Epub 2021 Sep 9.

Backbone

• Vector backbone: pET45b(+)
• Backbone size w/o insert (bp): 5062
• Total vector size (bp): 6760
• Vector type: Bacterial Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AwaIscB
• Alt name: IscB(HEARO)
• Species: Allochromatium warmingii, Brachypodium distachyon
• Insert Size (bp): 1608
• GenBank ID: SDX90858.1
• Promoter: T7
• Tag / Fusion Protein:
  • 14xHis-bdSUMO tag (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • phs0806-pet45b-his14-bdsumo-awaiscb.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry