MF87_PB-EF1a-TET3G-P2A-ERT2-Gal4-BGHpA
(Plasmid
#176630)
-
PurposeIntegrated in CHO-K1 to make parental polyclonal lines for all other MultiFate cell lines
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 176630 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonePiggyBac transposon system
-
Backbone manufacturerSystem Biosciences
-
Vector typeSynthetic Biology
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameEF1a-TET3G-P2A-ERT2-Gal4-BGHpA
-
SpeciesSynthetic
-
Insert Size (bp)4210
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
MF87_PB-EF1a-TET3G-P2A-ERT2-Gal4-BGHpA was a gift from Michael Elowitz (Addgene plasmid # 176630 ; http://n2t.net/addgene:176630 ; RRID:Addgene_176630) -
For your References section:
Synthetic multistability in mammalian cells. Zhu R, Del Rio-Salgado JM, Garcia-Ojalvo J, Elowitz MB. Science. 2022 Jan 21;375(6578):eabg9765. doi: 10.1126/science.abg9765. Epub 2022 Jan 21. 10.1126/science.abg9765 PubMed 35050677
Map uploaded by the depositor.
