|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17665||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameCx43 3'UTR
SpeciesM. musculus (mouse)
Entrez GeneGja1 (a.k.a. AU042049, AW546267, Cnx4, Cnx43, Cx43, Cx43al, Cx43alpha1, Cxnk1, Gja-, Gja-1, Npm1, conne, connexin43)
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
3'UTR of Mus musculus Gja1 (Cx43). For in situ riboprobe.
Anti-Sense: use SP6 and cut with ClaI.
Sense: use T7 and cut with HindIII.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p43BgI was a gift from Cecilia Lo (Addgene plasmid # 17665 ; http://n2t.net/addgene:17665 ; RRID:Addgene_17665)