Purpose(Empty Backbone) Expression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||176678||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeInsect Expression ; CRISPR
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Cloning of sgRNA targeting sequence into BbsI cassette.
Please visit https://www.biorxiv.org/content/10.1101/2021.03.29.437496v2 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLib6.4B-Consensus was a gift from Norbert Perrimon (Addgene plasmid # 176678 ; http://n2t.net/addgene:176678 ; RRID:Addgene_176678)
For your References section:Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos. Viswanatha R, Mameli E, Rodiger J, Merckaert P, Feitosa-Suntheimer F, Colpitts TM, Mohr SE, Hu Y, Perrimon N. Nat Commun. 2021 Nov 24;12(1):6825. doi: 10.1038/s41467-021-27129-3. 10.1038/s41467-021-27129-3 PubMed 34819517