|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17668||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)3300
Entrez GeneGja1 (a.k.a. AU042049, AW546267, Cnx43, Cx43, Cx43alpha1, Gja-1, Npm1, connexin43)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
For in situ riboprobe.
Anti-sense: use T7; cut with BamHI or HindIII.
Sense: use Sp6; cut with XbaI.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p132 was a gift from Cecilia Lo (Addgene plasmid # 17668 ; http://n2t.net/addgene:17668 ; RRID:Addgene_17668)