pcDNA6.2-YFP-CWT
(Plasmid #176689)

• Purpose: Fluorescent reporter for phagosomal escape of Staphylococcus aureus
• Depositing Lab: Martin Fraunholz
• Publication: Blattner et al PLoS Pathog. 2016 Sep 15;12(9):e1005857. doi: 10.1371/journal.ppat.1005857. eCollection 2016 Sep.

Backbone

• Vector backbone: pcDNA6.2
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cell wall-targeting domain of Lysostaphin
• Alt name: CWT
• Species: Aequorea victoria (modified), Staphylococcus simulans
• Insert Size (bp): 719
• Mutation: deletion of protase domain of Lysostaphin
• Promoter: CMV
• Tag / Fusion Protein:
  • YFP (N terminal on backbone)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: TK-pA-R

Resource Information

• Supplemental Documents:
  • pcDNA6.2 N-YFP-CWT.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry