pLV-hPLXNB2_PGK_Neo
(Plasmid #176848)

• Purpose: lentiviral vector for constitutive PLXNB2 overexpression
• Depositing Lab: Roland Friedel
• Publication: Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi: 10.1038/s41467-021-26296-7.

Backbone

• Vector backbone: pLENTI PGK Neo DEST
• Backbone manufacturer: Eric Campeau & Paul Kaufman (Addgene plasmid # 19067)
• Backbone size w/o insert (bp): 9778
• Vector type: Lentiviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hPLXNB2
• Species: H. sapiens (human)
• Insert Size (bp): 5517
• Mutation: full length PLXNB2
• Entrez Gene: PLXNB2 (a.k.a. MM1, Nbla00445, PLEXB2, dJ402G11.3)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: PGK-F

Resource Information

• Supplemental Documents:
  • 3-pLV-hPLXNB2_PGK_Neo.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is the wild-type full length cDNA sequence for PLXNB2. This plasmid is similar to Addgene plasmid # 86237, but is not CRISPR-resistant.