UBQLN2_450-624W
(Plasmid #176852)

• Purpose: Expresses human UBQLN2 construct 450-624 with added C-terminal W in bacterial cells
• Depositing Lab: Carlos Castañeda
• Publication: Dao et al Mol Cell. 2018 Mar 15;69(6):965-978.e6. doi: 10.1016/j.molcel.2018.02.004. Epub 2018 Mar 8.

Backbone

• Vector backbone: pET24b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5309
• Total vector size (bp): 5768
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: UBQLN2_450C_W
• Species: H. sapiens (human)
• Insert Size (bp): 534
• Mutation: deletion of residues 1-449 and addition of C-terminal W
• GenBank ID: NM_013444
• Entrez Gene: UBQLN2 (a.k.a. ALS15, CHAP1, DSK2, HRIHFB2157, N4BP4, PLIC2)
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 term

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Addgene plasmid #8661 (deposited by Peter Howley)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry