pEPQD1CB0871
(Plasmid #177087)

• Purpose: MoClo Level 1, position 2, transcriptional unit for transient expression of secologanin synthase (CrSLS1) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31
• Depositing Lab: Nicola Patron
• Publication: Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.

Backbone

• Vector backbone: pICH47742
• Vector type: Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: secologanin synthase (CrSLS1) from Catharanthus roseus
• Alt name: P2_4X opattB-TMV-CrSLS-nosterm
• Species: Other

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: CTGGTGGCAGGATATATTGTGGTG
• 3′ sequencing primer: GAACCCTGTGGTTGGCATGCACATAC

Resource Information

• Supplemental Documents:
  • pEPQD1CB0871.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry