wg186Fluc
(Plasmid
#177436)
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PurposeIn vitro synthesis of an mRNA encoding Firefly luciferase with A[62] tail.
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Depositing Lab
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Depositing OrganizationMassachusetts Institute of Technology
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 177436 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 177436-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 177436-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonewg186Fluc
- Backbone size w/o insert (bp) 2821
- Total vector size (bp) 4599
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Vector typeLuciferase ; For in vitro transcription
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFirefly luciferase
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Alt nameFluc
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SpeciesSynthetic; Photinus pyralis
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Insert Size (bp)1778
- Promoter T7
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Tag
/ Fusion Protein
- None
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Nco1 (not destroyed)
- 3′ cloning site Ecl136II (unknown if destroyed)
- 5′ sequencing primer TAATACGACTCACTATAG
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byWendy Gilbert, Yale University
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 177436-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 177436-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
wg186Fluc was a gift from Wendy Gilbert (Addgene plasmid # 177436 ; http://n2t.net/addgene:177436 ; RRID:Addgene_177436) -
For your References section:
Alternative transcription start site selection leads to large differences in translation activity in yeast. Rojas-Duran MF, Gilbert WV. RNA. 2012 Dec;18(12):2299-305. doi: 10.1261/rna.035865.112. Epub 2012 Oct 25. 10.1261/rna.035865.112 PubMed 23105001
