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Addgene

mCD8-GFP in pBS
(Plasmid #17745)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17745 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBS
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mouse CD8-GFP
  • Alt name
    mCD8-GFP
  • Alt name
    CD8a
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1350
  • Entrez Gene
    Cd8a (a.k.a. BB154331, Ly-2, Ly-35, Ly-B, Lyt-2)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    mCD8 construct from Jane Parnes

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCD8-GFP in pBS was a gift from Liqun Luo (Addgene plasmid # 17745 ; http://n2t.net/addgene:17745 ; RRID:Addgene_17745)
  • For your References section:

    Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis. Lee T, Luo L. Neuron. 1999 Mar . 22(3):451-61. 10.1016/S0896-6273(00)80701-1 PubMed 10197526