pEGFP-HA3-STONIN1
(Plasmid
#177580)
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PurposeExpresses Stonin1 in mammalian cells
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Depositing Lab
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Depositing OrganizationNational Institute of Child Health and Human Development (NICHD)
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 177580 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 177580-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 177580-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneEGFP
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSTONIN1
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SpeciesH. sapiens (human)
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Insert Size (bp)2200
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MutationV334A- please see depositor comments
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Tags
/ Fusion Proteins
- GFP (N terminal on insert)
- 3XHA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Kpn1 (unknown if destroyed)
- 3′ cloning site BamH1 (unknown if destroyed)
- 5′ sequencing primer GFP-Fward
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note there is a single mutation V334A compared to stonin-1 [Homo sapiens] NP_001185524.1. Depositor is unsure if this will affect plasmid function. We recommend additional diagnostic tests before using this plasmid.
DNA (Catalog # 177580-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 177580-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-HA3-STONIN1 was a gift from Juan Bonifacino (Addgene plasmid # 177580 ; http://n2t.net/addgene:177580 ; RRID:Addgene_177580) -
For your References section:
Stonin 2: an adaptor-like protein that interacts with components of the endocytic machinery. Martina JA, Bonangelino CJ, Aguilar RC, Bonifacino JS. J Cell Biol. 2001 May 28;153(5):1111-20. doi: 10.1083/jcb.153.5.1111. 10.1083/jcb.153.5.1111 PubMed 11381094
