pEGFP-HA3-STONIN1
(Plasmid #177580)

• Purpose: Expresses Stonin1 in mammalian cells
• Depositing Lab: Juan Bonifacino
• Publication: Martina et al J Cell Biol. 2001 May 28;153(5):1111-20. doi: 10.1083/jcb.153.5.1111.

Backbone

• Vector backbone: EGFP
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: STONIN1
• Species: H. sapiens (human)
• Insert Size (bp): 2200
• Mutation: V334A- please see depositor comments
• Tags / Fusion Proteins:
  • GFP (N terminal on insert)
  • 3XHA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn1 (unknown if destroyed)
• 3′ cloning site: BamH1 (unknown if destroyed)
• 5′ sequencing primer: GFP-Fward

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note there is a single mutation V334A compared to stonin-1 [Homo sapiens] NP_001185524.1. Depositor is unsure if this will affect plasmid function. We recommend additional diagnostic tests before using this plasmid.