pETDuet1-MBP-NAAEF-PNR(217-410 C275)-His6
(Plasmid #177849)

• Purpose: Bacterial Expression of PNR
• Depositing Lab: Karsten Melcher
• Publication: Tan et al PLoS One. 2013 Sep 12;8(9):e74359. doi: 10.1371/journal.pone.0074359. eCollection 2013.

Backbone

• Vector backbone: pETDuet1
• Backbone manufacturer: Novagen
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: photoreceptor cell-specific nuclear receptor
• Alt name: NR2E3 (nuclear receptor subfamily 2, group E, member 3)
• Species: H. sapiens (human)
• Entrez Gene: NR2E3 (a.k.a. ESCS, PNR, RNR, RP37, rd7)
• Tags / Fusion Proteins:
  • MBP (N terminal on insert)
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pET Upstream
• 3′ sequencing primer: DuetDOWN1

Resource Information

• Supplemental Documents:
  • pETDuet-MBP-NAAEFlinker-PNR(217-410)-H6.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry