pcDNA5-FRT/TO-N-mEGFP-PARP11
(Plasmid
#177998)
-
PurposeExpresses N-terminally mEGFP-tagged PARP11
-
Depositing Lab
-
Depositing OrganizationUniversitaetsklinikum Aachen
Located in Germany -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 177998 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 177998-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 177998-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepcDNA5/FRT/TO/N-mEGFP
- Total vector size (bp) 6936
-
Vector typeMammalian Expression
-
Selectable markersHygromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namePARP11
-
SpeciesH. sapiens (human)
-
Entrez GenePARP11 (a.k.a. ARTD11, C12orf6, MIB006)
-
Tag
/ Fusion Protein
- mEGFP (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer EGFP-C
- (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2022.02.22.481411 for bioRxiv preprint.
DNA (Catalog # 177998-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 177998-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA5-FRT/TO-N-mEGFP-PARP11 was a gift from Karla Feijs (Addgene plasmid # 177998 ; http://n2t.net/addgene:177998 ; RRID:Addgene_177998) -
For your References section:
Protein and RNA ADP-ribosylation detection is influenced by sample preparation and reagents used. Weixler L, Ikenga NJ, Voorneveld J, Aydin G, Bolte TM, Momoh J, Butepage M, Golzmann A, Luscher B, Filippov DV, Zaja R, Feijs KL. Life Sci Alliance. 2022 Nov 11;6(1):e202201455. doi: 10.26508/lsa.202201455. 10.26508/lsa.202201455 PubMed 36368907
